题   目：Enhanced Brownian Ratchet Molecular Separation using a Self-spreading Lipid Bilayer
报告人：Prof. Kei Murakoshi
单   位：Department of Chemistry, Faculty of Science, Hokkaido University
时   间：2012年4月27日（星期五）上午9时30分
地   点：化学楼三楼报告厅
联系人：赵健伟 教授

Abstract
      A novel approach is proposed for two-dimensional molecular separation based on the Brownian ratchet mechanism by use of a self-spreading lipid bilayer as both molecular transport and separation medium. In addition to conventional diffusivity-dependence on the ratchet separation efficiency, the difference in the intermolecular interactions between the target molecules and the lipid bilayer is also incorporated as a new separation factor in the present self-spreading ratchet system. Spreading at the gap between two ratchet obstacles causes a local change in the lipid density at the gap. This effect produces an additional opportunity for a molecule to be deflected at the ratchet obstacle and thus causes an additional angle shift. This enables the separation of molecules with the same diffusivity but with different intermolecular interaction between the target molecule and surrounding lipid molecules. Here we demonstrate this aspect by using Cholera toxin subunit B (CTB) - ganglioside GM1 (GM1) complexes with different configurations. Mechanism of the separation is also discussed based on the simulation carried out by the random walk program developed by Prof. J. Zhao’s group in Nanjing University. The present results will unlock a new strategy for two-dimensional molecular manipulation with ultra-small devices.

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