生命分析化学国家重点实验室

      Porous polymer monoliths for application as stationary phases for HPLC and supports for immobilization of enzymes

      Frantisek Svec

      The Molecular Foundry, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8139

      Porous polymer monoliths are now over 20 years old. They have already been used in numerous applications such as in HPLC, capillary electrochromatography, gas chromatography, ion chromatography, solid phase extraction, and enzyme immobilization. Their well known advantages include ease of the preparation, robustness, high permeability to flow, mass transfer via convection, and a vast variety of chemistries. This lecture will present examples confirming that the first generation monolithic columns are excellent stationary phase for the separation of large molecules. These monoliths are also suitable for immobilization of enzymes which will be demonstrated with protein digestion and de-glycosylation in aqueous environment as well as with reesterification of fatty acids in organic solvent.

      Although very useful in some applications, the first generation monoliths exhibit a small surface area since they do not include any mesopores. This lack makes them unsuitable for the separation of small molecules in isocratic mode. Therefore, we have recently developed monoliths of the second generation using a new two-step approach to the control of porous properties that includes hypercrosslinking reaction. Our technique now enables the preparation of porous polymer monoliths with an unprecedented surface area of up to 600 m2/g. Their applications will be demonstrated with both the highly efficient rapid separations of small molecules and size exclusion chromatography.

BIOGRAPHICAL SKETCH

      Frantisek Svec received both degrees B.S. in chemistry and Ph.D. degree in polymer chemistry from the Institute of Chemical Technology, Prague (Czech Republic) in 1965 and 1969, respectively. In 1976 he joined the Institute of Macromolecular Chemistry of the Czechoslovak Academy of Sciences where he was promoted through the ranks to the Head of Department and the Scientific Secretary of the Institute. He accepted an offer and joined faculty at Cornell University in 1992. Since 1997, he is appointed at the University of California, Berkeley. He also was a visiting professor of analytical chemistry at the University of Innsbruck, Austria, and currently works as Facility Director in the Molecular Foundry of the Lawrence Berkeley National Laboratory.  Dr. Svec authored almost 400 scientific publications, he edited 2 books, and authored 75 patents. He is Editor-in-Chief of the Journal of Separation Science and member of editorial boards of a number of renowned journals including Journal of Chromatography A, Electrophoresis, and Chinese Journal of Chromatography. In 2003 he was elected President of CASSS (formerly California Separation Science Society).  In 2005 he was awarded with M.J.E. Golay Medal in Chromatography and EAS Award for Achievements in Separation Science. In 2006, he obtained in Sweden Honorary doctorate of philosophy and in 2009 Honorary professorship in Dalian, China. He has been selected as the 2008 recipient of the ACS Award in Chromatography and 2009 Dal Nogare award. F. Svec is best known for his research in the area of macroporous polymers in different shapes such as beads, flat sheets, and, in particular, monoliths. His studies involve use of these materials in numerous applications including liquid chromatography, electrochromatography, supports for solid phase chemistry, enzyme immobilization, and microfluidics.